A multisystemic illness that affects both respiratory and non-respiratory organs, coronavirus disease 2019 (COVID-19). The SARS-CoV-2 virus has been linked in studies to a number of hematological disorders, but little is known about how the virus enters different organs.
Researchers used the murine hepatitis virus (MHV)-A59 model, a prototypical CoV in a naturally occurring host, to examine the relationship between viral organotropism and clinical symptoms.
Viral infectivity analysis and quantitative reverse-transcription-polymerase chain reaction (RT-qPCR) were carried out. We compared the plasma and RBC-enriched fractions.
To further explore the possibility of interaction between heme (and heme-associated molecules) and the spike protein of the murine hepatitis virus, computational in silico docking research was carried out.
37 individuals with COVID-19 who had their sera and nasopharyngeal swabs validated by RT-qPCR were collected. Ribonucleic acid (RNA) from SARS-CoV-2 was isolated. Eight people who died as a result of COVID-19 had their autopsies performed, and tissues from their kidneys, hearts, lungs, and liver were taken. For the in vivo tests, BALB/cJ mice were employed. They were intraperitoneally injected with the MHV virus and given either hemin or chloroquine alone or in combination.
For RT-qPCR and SARS-CoV-2 particle characterization, murine lung, liver, heart, kidney, spleen, pancreas, and brain tissues were acquired. Before and after infection, blood samples were taken for hematological and biochemistry-related evaluations.
