Researchers used the murine hepatitis virus (MHV)-A59 model, a prototypical CoV in a naturally occurring host, to examine the relationship between viral organotropism and clinical symptoms.
Viral infectivity analysis and quantitative reverse-transcription-polymerase chain reaction (RT-qPCR) were carried out. We compared the plasma and RBC-enriched fractions.
To further explore the possibility of interaction between heme (and heme-associated molecules) and the spike protein of the murine hepatitis virus, computational in silico docking research was carried out.
37 individuals with COVID-19 who had their sera and nasopharyngeal swabs validated by RT-qPCR were collected. In order to identify SARS-CoV-2 both qualitatively and quantitatively, ribonucleic acid (RNA) from the virus was isolated.
Eight people who died as a result of COVID-19 had their autopsies performed, and tissues from their kidneys, hearts, lungs, and liver were taken. In vivo tests were performed on BALB/cJ mice that were intraperitoneally infected with the MHV injection, and individually and in conjunction with hemin and chloroquine treatments.
For RT-qPCR and SARS-CoV-2 particle characterization, murine lung, liver, heart, kidney, spleen, pancreas, and brain tissues were acquired. Before and after infection, blood samples were taken for hematological and biochemistry-related evaluations.
Plaque tests were run to determine the viability of SARS-CoV-2 particles. The scientists matched the findings to actual data on SARS-CoV-2 positive people, including autopsies of people who had died with COVID-19.
